Dual-Lumi Dual-Luciferase Reporter Gene Assay Kit

A sequential dual-luciferase reporter kit that reads firefly luciferase first and Renilla luciferase second from the same lysate. Each kit covers 100 tests.
How it works
In a dual-reporter assay, one luciferase reports on your promoter or pathway of interest and the other, expressed from a control vector, normalises for transfection efficiency and cell number. Firefly luciferase is measured first, then a second reagent quenches that signal and starts the Renilla reaction, so both readings come from one sample.
Key features
Sequential firefly then Renilla measurement
Per test: 20 µL lysate, 100 µL firefly reagent, 100 µL Renilla reagent
Lysis buffer 50 mL, two 10 mL reaction buffers and two 200 µL 50X substrates
100 tests per kit
How to use
Lyse transfected cells with the lysis buffer.
Add the firefly reagent to 20 µL of lysate and read the luminescence.
Add the Renilla reagent to the same well and read again, then normalise firefly to Renilla.
Specifications
Method: Dual-luciferase, sequential
Per test: 20 µL lysate + 100 µL firefly reagent + 100 µL Renilla reagent
Components: Lysis buffer 50 mL; 2 × 10 mL reaction buffer; 2 × 200 µL 50X substrate
Tests: 100
Catalog number: LN-G1701-100T