Mono-Lumi Renilla Luciferase Reporter Gene Assay Kit

A Renilla luciferase reporter kit for measuring reporter activity in transfected cells, including its use as an internal control. Each kit covers 100 tests.
How it works
Renilla luciferase is a reporter enzyme that produces light from its own substrate. Because it is a different enzyme from firefly luciferase, it is often expressed from a control vector alongside a firefly reporter to normalise for transfection efficiency, and it can also be used on its own as the main reporter.
Key features
Per test: 20 µL lysate and 100 µL working solution
Lysis buffer 50 mL, substrate (50X) 200 µL and buffer 10 mL
100 tests per kit
How to use
Lyse transfected cells with the lysis buffer.
Mix the substrate with the buffer to make the working solution.
Add 100 µL of working solution to 20 µL of lysate and read the luminescence.
Specifications
Method: Renilla luciferase
Per test: 20 µL lysate + 100 µL working solution
Components: Lysis buffer 50 mL; 50X substrate 200 µL; buffer 10 mL
Tests: 100
Catalog number: LN-G1703-100T