3D Cell Viability Assay Kit, ATP Luminescence, with Enhanced Lysis Buffer

A luminescent ATP assay built for 3D cultures. A single reagent with an enhanced lysis buffer measures viable cells in spheroids and gives a glow-type signal. The 10 mL pack covers about 100 wells of a 96-well plate.
How it works
Viable cells make ATP, and the amount of ATP is proportional to the number of living cells. The reagent lyses the cells, using an enhanced lysis buffer that helps it reach cells inside spheroids, and the released ATP drives a luciferase reaction that gives off light. Luminescence therefore reflects the number of viable cells in the 3D culture.
Key features
Single reagent with enhanced lysis buffer
Designed for 3D cultures such as spheroids
Glow-type luminescent signal
10 mL covers about 100 wells of a 96-well plate
How to use
Grow and treat 3D cultures in an opaque-walled multi-well plate.
Add the reagent and mix well so that the lysis buffer can penetrate the spheroids.
Read the luminescence on a plate luminometer.
Specifications
Method: ATP luminescence
Culture type: 3D
Signal: Glow-type
Size: 10 mL (about 100 wells, 96-well plate)
Catalog number: LN-G1615-10ML