Click-iT EdU-647 Cell Proliferation Assay Kit

An EdU cell proliferation assay kit with a far-red fluorescent dye (excitation 656 nm, emission 670 nm) for imaging, with Hoechst 33342 included for nuclear counterstaining. Each kit covers 100 tests.
How it works
EdU (5-ethynyl-2'-deoxyuridine) is a thymidine analogue that cells build into new DNA while they replicate. A fluorescent azide then binds the EdU in a click reaction, so cells that were making DNA light up. Because the detection does not need the DNA to be denatured, cell structure and other stains are better preserved than with BrdU methods.
Key features
Far-red fluorescence, excitation 656 nm and emission 670 nm
Hoechst 33342 included to stain all nuclei
Designed for imaging
100 tests per kit
How to use
Label proliferating cells with EdU in culture.
Fix and permeabilise the cells.
Run the click reaction with the fluorescent azide.
Counterstain nuclei with Hoechst 33342, then image.
Specifications
Method: EdU click-chemistry detection
Detection dye: Far-red, ex 656 nm / em 670 nm
Counterstain: Hoechst 33342 included
Application: Imaging
Tests: 100
Catalog number: LN-G1604