Calcein AM Cell Viability Assay Kit, Live-Cell Fluorescence, with Buffer

A fluorescence kit for detecting viable cells with Calcein AM, supplied with detection buffer. It comes in two sizes: 100 tests and 500 tests.
How it works
Calcein AM passes through the membrane of living cells. Inside, enzymes called esterases remove the AM groups, which traps green-fluorescent calcein in intact cells. Dead cells with damaged membranes do not retain the dye, so the green signal marks viable cells.
Key features
Calcein AM solution (1000X) plus detection buffer
100 tests: Calcein AM 10 µL + detection buffer 10 mL
500 tests: Calcein AM 50 µL + detection buffer 50 mL
How to use
Dilute the Calcein AM 1:1000 in detection buffer to make a working solution.
Add the working solution to the cells and incubate.
Read the green fluorescence with a microscope, plate reader or flow cytometer.
Specifications
Method: Calcein AM fluorescence
100 tests: Calcein AM 10 µL (1000X) + detection buffer 10 mL
500 tests: Calcein AM 50 µL + detection buffer 50 mL
Catalog numbers: LN-G1609-100T (100 tests), LN-G1609-500T (500 tests)