Click-iT EdU-488 Cell Proliferation Detection Kit

An EdU cell proliferation detection kit with a green fluorescent dye (488 nm channel) for imaging, with Hoechst 33342 included for nuclear counterstaining. Each kit covers 100 tests.
How it works
EdU (5-ethynyl-2'-deoxyuridine) is a thymidine analogue that cells build into new DNA while they replicate. A fluorescent azide then binds the EdU in a click reaction, so cells that were making DNA light up. Because the detection does not need the DNA to be denatured, cell structure and other stains are better preserved than with BrdU methods.
Key features
Green fluorescence detected in the 488 nm channel
Hoechst 33342 included to stain all nuclei
Suitable for imaging and flow cytometry
100 tests per kit
How to use
Label proliferating cells with EdU in culture.
Fix and permeabilise the cells.
Run the click reaction with the fluorescent azide.
Counterstain nuclei with Hoechst 33342, then image.
Specifications
Method: EdU click-chemistry detection
Detection dye: Green, 488 nm channel
Counterstain: Hoechst 33342 included
Application: Imaging and flow cytometry
Tests: 100
Catalog number: LN-G1601